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KMID : 0525720180230010036
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Journal of Chitin and Chitosan
2018 Volume.23 No. 1 p.36 ~ p.43
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Antioxidant Activities of Crude Polysaccharides from Glehniae Radix Root using Pressurized Extraction
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Kim Ju-Hyeon
Gu Yul-Ri
Hong Joo-Heon
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Abstract
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This study was conducted to establish antioxidant activities of crude polysaccharides from Glehniae Radix root using pressurized extraction. The crude polysaccharides were isolated from Glehniae Radix roots by extracting at 150 kPa, 121oC for 30 min (PE30), 60 min (PE60), 120 min (PE120) and 180 min (PE180). The yield of crude polysaccharides (HE, PE30, PE60, PE120, PE180) were 24.60~31.14%. The total sugar and protein contents of PE180 were 57.09 g/100 g and 15.41 g/100 g, respectively. The main components in the free sugar of polysaccharide from Glehniae Radix root were fructose and glucose at 82.72~89.03 mg/100 g, 52.98~54.39 mg/100 g, respectively. The total polyphenol and flavonoid contents of PE60 were 7.95 g/100 g and 6.49 g/100 g, respectively. The DPPH and ABTS radical scavenging activities of PE60 at 5,000 μg/mL were 45.31% and 61.12%, respectively. The superoxide radical scavenging and FRAP activity of PE60 at 5,000 μg/mL were 56.08% and 421.22 μM, respectively. The PE60 extract was better than other extracts on protective effects against oxidative stress in L-132 cells (83.35%). In conclusion, we provided experimental evidence that crude polysaccharide from Glehniae Radix root have potential as functional food materials.
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KEYWORD
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Glehniae Radix root, Crude polysaccharide, Pressurized extraction, Antioxidant activities
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